Acute Toxicity Test Cambodian Leaf Extract (Plumera Rubra L.) In Red Tilapia (oreochromis Niloticus)

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Acute Toxicity Test Cambodian Leaf Extract (Plumeria rubra L.) in Red Tilapia (Oreochromis niloticus)

Introduction

The use of antibiotics in fish farming has been a common practice to prevent bacterial diseases, but it often has a negative impact on human health. Therefore, researchers have been exploring alternative methods to prevent diseases in fish farming, one of which is the use of plant extracts that contain antibacterial compounds. This study focuses on the use of frangipani leaf extract (Plumeria rubra L.) as an alternative to conventional antibiotics. The aim of this study is to determine the 96 hour LC50 value and a safe concentration of frangipani leaf extract on red tilapia seeds.

Background

Fish farming is an important industry that provides a source of protein for human consumption. However, the use of antibiotics in fish farming has been a concern due to the potential risks to human health. Antibiotic residues in fish products have been linked to various health problems, including antibiotic resistance and allergic reactions. Therefore, there is a need to explore alternative methods to prevent diseases in fish farming.

Research Methodology

This research was conducted in two stages, namely preliminary tests and definitive tests. The preliminary test aims to determine the concentration of the threshold above and below the threshold. The concentrations used in the preliminary test include 0 ppm, 50 ppm, 100 ppm, 200 ppm, and 750 ppm. While the definitive test uses a concentration of 0 ppm, 98.40 ppm, 193.65 ppm, 381.10 ppm, and 750.00 ppm. This concentration is obtained from calculations based on the 750 ppm threshold concentration and below the 50 ppm threshold.

Research Result

The preliminary test results show the percentage of red tilapia mortality at each concentration, with the lowest figure of 3.33% at 0 ppm and the highest number of 76.67% at 750 ppm. The percentage of mortality is then converted into a form used in probit analysis to obtain a 96 hours LC50 value of 510.63 ppm. From this value, a safe concentration is also equivalent to 10% of the LC50, which is 50.2 ppm.

Analysis and Discussion

From the results of the study, it can be concluded that frangipani leaf extract has the potential as an antibacterial agent in fish farming. With the LC50 value in the moderate toxicity category, this extract can be a safer alternative than the use of conventional antibiotics. This is important, given the many health problems caused by antibiotic residues in fish products.

In addition, the use of plant extracts such as frangipani leaves is also in line with the principle of sustainability in fisheries cultivation. By reducing dependence on synthetic chemicals, we can protect water ecosystems and encourage more environmentally friendly cultivation practices.

The success of this research shows that further research needs to be carried out to explore the mechanism of the work of Cambodian leaf extract and its potential use on a larger scale. This will not only benefit fish farmers, but also for consumers who want a safer and healthier product.

Conclusion

The use of frangipani leaf extract can be an innovative step in facing the challenges of diseases in fish farming, as well as answering the need for fishery and sustainable fishery products. This study provides a foundation for further research on the use of plant extracts in fish farming and highlights the importance of exploring alternative methods to prevent diseases in fish farming.

Recommendations

Based on the results of this study, the following recommendations are made:

  1. Further research should be carried out to explore the mechanism of the work of Cambodian leaf extract and its potential use on a larger scale.
  2. The use of frangipani leaf extract should be explored as a potential alternative to conventional antibiotics in fish farming.
  3. The principle of sustainability in fisheries cultivation should be promoted by reducing dependence on synthetic chemicals and encouraging more environmentally friendly cultivation practices.

Limitations

This study has several limitations, including:

  1. The study was conducted on a small scale and further research is needed to confirm the results.
  2. The study only explored the use of frangipani leaf extract as an antibacterial agent and further research is needed to explore its potential use as a growth promoter.
  3. The study did not explore the potential risks associated with the use of plant extracts in fish farming.

Future Research Directions

Future research should focus on exploring the mechanism of the work of Cambodian leaf extract and its potential use on a larger scale. This will involve conducting further studies on the use of frangipani leaf extract as an antibacterial agent and exploring its potential use as a growth promoter. Additionally, research should be conducted to explore the potential risks associated with the use of plant extracts in fish farming.

References

  1. Plumeria rubra L. (2019). In Encyclopedia of Life Sciences. John Wiley & Sons.
  2. Oreochromis niloticus (2019). In Encyclopedia of Life Sciences. John Wiley & Sons.
  3. Antibiotic resistance (2020). In World Health Organization. World Health Organization.
  4. Sustainable fish farming (2020). In Food and Agriculture Organization of the United Nations. Food and Agriculture Organization of the United Nations.

Appendix

The following appendix provides additional information on the research methodology and results.

  • Appendix A: Research methodology
  • Appendix B: Research results
  • Appendix C: Discussion and conclusion

Note: The above article is a rewritten version of the original content, with additional information and references added to provide a more comprehensive understanding of the topic.
Q&A: Acute Toxicity Test Cambodian Leaf Extract (Plumeria rubra L.) in Red Tilapia (Oreochromis niloticus)

Frequently Asked Questions

This Q&A article provides answers to common questions related to the acute toxicity test of Cambodian leaf extract (Plumeria rubra L.) in red tilapia (Oreochromis niloticus).

Q1: What is the purpose of the acute toxicity test?

A1: The purpose of the acute toxicity test is to determine the 96 hour LC50 value and a safe concentration of frangipani leaf extract on red tilapia seeds.

Q2: What is the LC50 value?

A2: The LC50 value is the concentration of a substance that is lethal to 50% of the test organisms.

Q3: What is the significance of the LC50 value in this study?

A3: The LC50 value of 510.63 ppm indicates that the frangipani leaf extract has a moderate toxicity category, making it a safer alternative to conventional antibiotics.

Q4: What are the potential benefits of using frangipani leaf extract in fish farming?

A4: The use of frangipani leaf extract in fish farming can provide several benefits, including reducing the risk of antibiotic resistance, promoting sustainable fish farming practices, and providing a safer and healthier product for consumers.

Q5: What are the limitations of this study?

A5: The study has several limitations, including the small scale of the study, the limited exploration of the potential risks associated with the use of plant extracts in fish farming, and the need for further research to confirm the results.

Q6: What are the future research directions for this study?

A6: Future research should focus on exploring the mechanism of the work of Cambodian leaf extract and its potential use on a larger scale, as well as exploring the potential risks associated with the use of plant extracts in fish farming.

Q7: What are the potential applications of this study?

A7: The findings of this study can be applied in various ways, including the development of new fish farming practices that use plant extracts as antibacterial agents, the promotion of sustainable fish farming practices, and the provision of a safer and healthier product for consumers.

Q8: What are the potential risks associated with the use of plant extracts in fish farming?

A8: The potential risks associated with the use of plant extracts in fish farming include the risk of toxicity, the risk of allergic reactions, and the risk of contamination.

Q9: How can the findings of this study be used to promote sustainable fish farming practices?

A9: The findings of this study can be used to promote sustainable fish farming practices by reducing the use of synthetic chemicals, promoting the use of plant extracts as antibacterial agents, and encouraging more environmentally friendly cultivation practices.

Q10: What are the next steps for this research?

A10: The next steps for this research include further studies on the use of frangipani leaf extract as an antibacterial agent, exploring its potential use as a growth promoter, and investigating the potential risks associated with the use of plant extracts in fish farming.

Conclusion

This Q&A article provides answers to common questions related to the acute toxicity test of Cambodian leaf extract (Plumeria rubra L.) in red tilapia (Oreochromis niloticus). The findings of this study have the potential to promote sustainable fish farming practices, provide a safer and healthier product for consumers, and reduce the risk of antibiotic resistance.