Induction Of Repair Dentin Formation In Reversible Pulpitis Due To Mechanical Trauma With Hydrogel Chitosan Blangkas (tachypellus Gigas) Nanoparticles And Yellow Stem Extract (in Vivo)
Induction of Repair Dentin Formation in Reversible Pulpitis with Blangkas Chitosan Hydrogel and Kemuning Stem Extract: In Vivo Study
Introduction
Reversible pulpitis due to mechanical trauma is a common challenge faced by dentists worldwide. The dental pulp, a vital connective tissue in the teeth, has limited regeneration capabilities due to its position in the terneralized hard tissue. When the pulp is injured, it often experiences inflammation, which can lead to more severe conditions if not treated properly. This study aims to explore the effectiveness of chitosan hydrogels made from blangkas (tachypellus gigas) and yellow stem extract in stimulating the formation of repair dentin in teeth with reversible pulpitis.
The Importance of Dental Pulp
The dental pulp plays a vital role in maintaining the health and function of teeth. However, it is also a vulnerable tissue that can be easily injured due to various factors such as iatrogenic factors, inappropriate dental care, and mechanical trauma. When the pulp is injured, it often undergoes an inflammatory process, which can lead to more severe conditions if not treated properly. Therefore, it is essential to find effective treatments that can stimulate the formation of repair dentin in teeth with reversible pulpitis.
The Potential of Chitosan and Kemuning
Chitosan, a natural and non-toxic polymer, has been widely used in health research due to its regenerative and anti-inflammatory abilities. On the other hand, Kemuning (Murraya Paniculata) is a plant that is often found in Indonesia and is known to have good anti-inflammatory properties. The combination of these two materials is expected to make a positive contribution to the formation of repair dentin. Chitosan can provide a scaffold for cell growth and differentiation, while Kemuning can provide anti-inflammatory properties that can help to reduce inflammation and promote healing.
Research Methodology
This study uses a post-test only group design with the in Vivo approach. The sample used is 27 molar of the maxillary of wistar mice, which is divided into three treatment groups: chitosan hydrogel and yellow stem extract, Ca (OH) â‚‚, and negative control groups. After preparing the tooth to perforation, the test material is applied, and then the teeth are pounded with a modified glass ionomer cement (RM-GIC) resin. Rats are reduced according to the group of days (7, 14, and 30 days), and teeth are extracted for further analysis.
Histopathological Analysis
Extracted teeth are then processed for histopathological analysis by staining hematoxylin-eosin (HE) to observe inflammatory cells and repair dentin formation under 400x magnification. Data testing was carried out by the Kruskall-Wallis and Mann-Whitney test to determine the significance between different groups.
Results and Analysis
The results showed that the use of hydrogel chitosan and yellow stem extract had a significant effect in stimulating the formation of repair dentin compared to Ca (OH) â‚‚. The Kruskall-Wallis test shows the value of p = 0.03 for chitosan hydrogel and yellow stem extract and p = 0.034 for Ca (OH) â‚‚, which shows a significant difference in treatment. The Mann-Whitney test also shows a significant difference between chitosan hydrogel and yellow and Ca (OH) â‚‚, with a value of p = 0.026.
Conclusion
Based on this study, it can be concluded that chitosan hydrogels from blangkas and yellow stem extracts have the potential to be an alternative treatment to stimulate the formation of repair dentin in teeth with reversible pulpitis. The use of natural ingredients not only provides a safer and more effective solution but can also be a new approach in dental treatment. Further research is needed to explore the mechanism of action and long-term effectiveness of this therapy, as well as the potential application in daily dentistry practices.
Future Directions
This study provides a new direction for the treatment of reversible pulpitis. The use of natural ingredients such as chitosan and Kemuning can provide a safer and more effective solution compared to traditional treatments. Further research is needed to explore the mechanism of action and long-term effectiveness of this therapy. Additionally, the potential application of this therapy in daily dentistry practices should be explored.
Limitations
This study has some limitations. The sample size is relatively small, and the study only used wistar mice as the model. Further studies should be conducted to confirm the results and explore the potential application of this therapy in humans.
Conclusion
In conclusion, this study provides evidence that chitosan hydrogels from blangkas and yellow stem extracts have the potential to be an alternative treatment to stimulate the formation of repair dentin in teeth with reversible pulpitis. The use of natural ingredients not only provides a safer and more effective solution but can also be a new approach in dental treatment. Further research is needed to explore the mechanism of action and long-term effectiveness of this therapy, as well as the potential application in daily dentistry practices.
Q&A: Induction of Repair Dentin Formation in Reversible Pulpitis with Blangkas Chitosan Hydrogel and Kemuning Stem Extract
Q: What is reversible pulpitis, and why is it a challenge for dentists?
A: Reversible pulpitis is a condition where the dental pulp, a vital connective tissue in the teeth, experiences inflammation due to mechanical trauma or other factors. It is a challenge for dentists because the pulp has limited regeneration capabilities, and if not treated properly, it can lead to more severe conditions.
Q: What is the role of chitosan in this study?
A: Chitosan is a natural and non-toxic polymer that has been widely used in health research due to its regenerative and anti-inflammatory abilities. In this study, chitosan is used to create a hydrogel that can provide a scaffold for cell growth and differentiation, promoting the formation of repair dentin.
Q: What is the role of Kemuning stem extract in this study?
A: Kemuning (Murraya Paniculata) is a plant that is often found in Indonesia and is known to have good anti-inflammatory properties. In this study, Kemuning stem extract is used to provide anti-inflammatory properties that can help to reduce inflammation and promote healing.
Q: How was the study conducted?
A: The study used a post-test only group design with the in Vivo approach. The sample used was 27 molar of the maxillary of wistar mice, which was divided into three treatment groups: chitosan hydrogel and yellow stem extract, Ca (OH) â‚‚, and negative control groups. The teeth were prepared to perforation, and the test material was applied, followed by pounding with a modified glass ionomer cement (RM-GIC) resin.
Q: What were the results of the study?
A: The results showed that the use of hydrogel chitosan and yellow stem extract had a significant effect in stimulating the formation of repair dentin compared to Ca (OH) â‚‚. The Kruskall-Wallis test showed a significant difference in treatment, and the Mann-Whitney test also showed a significant difference between chitosan hydrogel and yellow and Ca (OH) â‚‚.
Q: What are the implications of this study?
A: This study provides evidence that chitosan hydrogels from blangkas and yellow stem extracts have the potential to be an alternative treatment to stimulate the formation of repair dentin in teeth with reversible pulpitis. The use of natural ingredients not only provides a safer and more effective solution but can also be a new approach in dental treatment.
Q: What are the limitations of this study?
A: This study has some limitations, including a relatively small sample size and the use of wistar mice as the model. Further studies should be conducted to confirm the results and explore the potential application of this therapy in humans.
Q: What are the future directions for this research?
A: Further research is needed to explore the mechanism of action and long-term effectiveness of this therapy, as well as the potential application in daily dentistry practices. Additionally, the potential application of this therapy in humans should be explored.
Q: Can this therapy be used in humans?
A: While this study provides promising results, further research is needed to confirm the safety and effectiveness of this therapy in humans. However, the use of natural ingredients such as chitosan and Kemuning makes it a promising approach for dental treatment.
Q: What are the potential benefits of this therapy?
A: The potential benefits of this therapy include a safer and more effective solution for treating reversible pulpitis, as well as a new approach in dental treatment. Additionally, the use of natural ingredients can reduce the risk of adverse reactions and promote healing.